How can we avoid this? Basically, we have two solutions to the problem of ice nucleation process in ice water extractions: the first one is flash freezing the plant material so very few crystals - and smaller - are formed. For this reason, this phenomenon is normally observed only when we perform a long first run or during the following runs in other words, this happens as the plant matter is defrosted during the ice water extraction process. However, as this plant matter starts defrosting the crystals blocking the pierced walls disappear, so the chlorophyll stored in the chloroplasts is released into the water and mixed with our resin glands, which is "dyed" dark green. But, how does this affect our fresh frozen material?Ĭhloroplast, x12000 (Picture: Bela Hausmann)ĭuring the first run, the plant material is still completely frozen, so the ice crystals formed in the tissues of the plant epidermis actually block those piercings and no chlorophyll is released into the water. The cell walls are also damaged and pierced by these crystals, releasing the chlorophyll stored in the thylakoids, inside the chloroplast. This phenomenon causes protoplasm - the inner part of the cell including the cytoplasm and the nucleus - dehydration, being severely damaged the cell collapses as the ice crystals expand. Since the fresh plant material is composed of approximately 80% water, ice crystals are soon formed inside the plant tissues, in the intracellular spaces, the xylem vessels and the stomal cavities. The surface of the plant tissue gets frosted from temperatures between -3✬ / -5✬. Unfortunately, most cannabis strains do not tolerate frost and are specially sensitive to this phenomenon. In this way, the damages do not depend as much on the minimum temperature as they do on whether if ice crystals are formed or not. This affects the plant tissues to a greater or lesser extent, depending on several factors: the natural resistance of the plant to frost, freezing and defrosting speeds or the minimum temperature reached. When we freeze fresh plant material (this also happens in Nature with frosts) very small ice crystals are formed in the plant tissues - phenomenon known as ice nucleation process - and damage both their intracellular (protoplasm) and extracellular structures. But, why does it happen and how to avoid it? When someone uses fresh frozen buds/trim and performs successive runs, it is not strange observing how the resin collected from the second and following runs is much darker than the resin from the first one, decreasing the quality of the resin extract. The ice nucleation process in frozen cannabis tissuesįrost precedes the ice nucleation process (Picture: Jun)Īs we have seen, freezing the plant material enables a faster and more efficient separation than not freezing it at all, although it does have a main disadvantage especially if we plan to make several runs with the same material. Let's see now what happens when we freeze fresh buds. This technique works well either for dry or fresh plant material, although it does have some cons when using fresh buds/trim and trying to make several runs. Thus, our resins have a much wider organoleptic profile.Īs we also discussed in our post on how to make water hash, freezing the plant material highly facilitates the extraction process, since trichome heads are removed much easily when they're frozen than when being, for example, at room temperature. As we already mentioned, the flavour of Fresh Frozen hashish is much closer to that of the plant than traditional hashish, since almost no degradation of Beta-Myrcene is produced. In this way, we get an extremely pure concentrate, both for the lack of adulterants (we'll never add any substance to improve the texture or effect of the resin) and contaminants, since the plant material is not shredded into small particles that contaminate the extract. In our article about the Fresh Frozen technique we already introduced some of the advantages of using fresh buds or trim when making water hash, such as maintaining a terpene profile much closer to the live plant or the fact that fresh plant material doesn't contaminate the extraction as dried buds/trim do. It is a variation on the traditional way of extracting cannabis resin with water and ice in which fresh frozen plant material is used. In recent years, the Fresh Frozen technique has become more and more popular among hashish makers. Making hashish without freezing the cannabis buds/trimįresh Chilled Separation, a variation on the Fresh Frozen technique.The ice nucleation process in frozen cannabis tissues.Fresh Chilled Separation, a variation on the Fresh Frozen technique.
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